Multiplex Real time PCR Kit for Monkeypox Virus and Central/West African Clade Typing

This kit is used for in vitro qualitative nucleic acid detection of Monkeypox Virus and the central/west African clade in lesion exudate samples, scab specimens, nasopharyngeal swab, oropharyngeal swab. Primer sets and FAM labeled probes are designed for specific detection of Monkeypox Virus, ROX labeled probe for the central African clade, CY5 labeled probe for the west African clade. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with VIC.